Unique in-plate liquid handling, patching and recording
IonFlux use a unique plate design that allows for all fluid activity to be conducted within the recording plate proper. Using flexible and user controlled pneumatic system, IonFlux provide high speed liquid exchange, continuous solution flow, and drug delivery without the use of an external liquid handler. The in-plate movement of solutions cancels typical ion channel assays problems such as stacking related drug cross contamination. The continuous flow system alleviates assays with slow acting or sticky compounds.
S i m p l e Y e t I n t u i t i v e P l a t e D e s i g n
IonFlux Plates are microfluidic devices used exclusively in the IonFlux System for automated patch clamp assays. They are integrated into SBS-standard well plate formats for convenience and compatibility with automated liquid handling instrumentation. Multiple plate formats are available which correspond to the IonFlux system configuration. Each well on the plate has a dedicated function as either a compound well, cell trapping well, cell inlet or cell outlet well.
Plates are divided into experimental patterns. Each pattern contains specified wells:
TWO Independent Trapping wells: These wells would contain intracellular solutions
Cell Inlet well: This well would contain the prepared cells in extracellular solution
Cell Outlet well: Acts as a waste well for the pattern
EIGHT independent compound wells: These wells would contain any combination of different compound solutions or dose response concentration gradient of the same compound. IonFlux software provides full control over these compound wells release and timing.
IonFlux plates come in two different standard designs, 96 well plates for use with all IonFlux system having 16 amplifiers and 384 well plates for use with all IonFlux systems with 64 amplifiers.
96 Well Plate
Used with IonFlux Mercury 16
384 Well Plate
Used with IonFlux Mercury HT
S i n g l e C e l l o r E n s e m b l e R e c o r d i n g s
IonFlux plates come in two different cell trapping and recording designs:
Single Cells: Using whole cell patch clamp technique and achieving giga-ohm seal recordings, IonFlux single cell plates are perfect for specialized assays requiring complete voltage/current control of an independent cell, mimicking manual patch clamp conditions while providing automated systems flexibility.
Ensemble cells: Just like single cell plates, ensemble plates also use whole cell patch clamp techniques but now with 20 cells in parallels setting a standard for consistency and throughput All IonFlux instruments can use either plate design with no hardware modifications.
You can order IonFlux consumables directly from Fluxion Biosciences
The fastest way to order is via a credit card using our online store.
If this is not convenient or you would like to schedule your order for a later date, you can contact us for a quote