An Advanced Automated Patch Clamp Protocol Design to Investigate Drug-Ion Channel Binding Dynamics
Traditional screening projects that utilize automated patch-clamp instruments often overlook crucial details about how drugs work, such as the dynamics of binding and unbinding, as well as the modulation of gating. This study aims to showcase that a more comprehensive analysis can be achieved without sacrificing throughput. By utilizing the cutting-edge microfluidics-based automated patch clamp, IonFlux Mercury, this customer developed a rapid assessment method to understand the mechanism of action of sodium channel inhibitors, including their kinetics of association and dissociation in different states.
Responsible for the rise of the action potential in neurons and cardiac muscle, sodium channels are important targets for drug research. Inhibitory drug interactions are investigated in safety pharmacology, pain research, and naturally-occurring toxin research. Although automated patch clamp systems have successfully studied sodium channels, many complicated assays stay elusive.
IonFlux Mercury can act as the missing link between HTS systems and the flexibility of manual patch clamp. Sodium channel IonFlux assays benefit from:
Continuously flowing solutions prolong assay stability
Short additions followed by controlled continuous wash of drugs provide direct calculation of binding kinetics
Precise temperature control provides physiological conditions for drug interactions
Nav 1.8 Lidocaine dose response
Nav 1.7 lidocaine dose response
Gigaseal single cell recordings of Nav 1.7
See What IonFlux Mercury can do for you
IonFlux Mercury is a next-generation APC family of system designed with unique in-plate fast solutions exchange and continuous flow. With simplified integrated operation and a plate reader design, IonFlux Mercury can fit into the smallest laboratory to the largest screening facility.