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At Fluxion, we’re passionate about delivering cell-based and cell-free solutions that facilitate the transformation of research discoveries into new ways to diagnose and treat patients. By characterizing molecular and cellular mechanisms of disease, Fluxion’s platforms help bridge the translational medicine gap, enabling rapid advances in disease research, drug discovery, and the development of diagnostic tests.

IonFlux Consummables

IonFlux Plates

Unique in-plate liquid handling, patching and recording

IonFlux uses a unique plate design that allows for all fluid activity to be conducted within the recording plate proper. Using flexible and user controlled pneumatic system, IonFlux provides high speed liquid exchange, continuous solution flow, and drug delivery without the use of an external liquid handler. The in-plate movement of solutions cancels typical ion channel assays problems such as stacking related drug cross contamination. The continuous flow system alleviates assays with slow acting or sticky compounds.

IonFlux plate design on the cover of Assay and Drug Development in 2012 

Assay Cover

IonFlux Plates are microfluidic devices used exclusively in the IonFlux System for automated patch clamp assays. They are integrated into SBS-standard well plate formats for convenience and compatibility with automated liquid handling instrumentation. Multiple plate formats are available which correspond to the IonFlux system configuration. Each well on the plate has a dedicated function as either a compound well, cell trapping well, cell inlet or cell outlet well.

Plates are divided into experimental patterns. Each pattern contains specified wells:

  • Two Independent trapping wells: These wells would contain intracellular solutions

  • Cell inlet well: This well would contain the prepared cells in extracellular solution

  • Cell outlet well: Acts as a waste well for the pattern

  • Eight independent compound wells: These wells would contain any combination of different compound solutions or dose response concentration gradient of the same compound. IonFlux software provides full control over these compound wells release and timing.

well+plate+diagram

 

IonFlux plates come in two different standard designs, 96 well plates for use with all lonFlux system having 16 amplifiers and 384 well plates for use with all lonFlux systems with 64 amplifiers or more.

IF Plates Underside

 

IonFlux plates come in two different cell trapping and recording designs:

  • Single Cells: Using whole cell patch clamp technique and achieving Giga-Ω seal recordings, lonFlux single cell plates are perfect for specialized assays requiring complete voltage/current control of an independent cell, mimicking manual patch clamp conditions while providing automated systems flexibility.
  • Ensemble cells: Just like single cell plates, ensemble plates also use whole cell patch clamp techniques but now with 20 cells in parallel, setting a standard for consistency and throughput.
All IonFlux instruments can use either plate design with no hardware modifications.

Single_vs_ensemble

 

Plate Types

IonFlux plates are shipped in boxes of 8 pieces. 

IMG_1210-1
IonFlux Plate 16
(ENSEMBLE)
PART# 910-0054

For lonFlux 16, lonFlux Classic 16, lonFlux Mercury 16.  Plates are standard SBS 96 wells. 

IMG_1210-1
IonFlux Plate 16
(SINGLE CELLS)
PART# 910-0058

For lonFlux 16, lonFlux Classic 16, lonFlux Mercury 16.  Plates are standard SBS 96 wells. 

IMG_1222-1
IonFlux Plate HT
(ENSEMBLE)
PART# 910-0055

For lonFlux HT, lonFlux Mercury HT, IonFlux Mercury ULTRA.  Plates are standard SBS 384 wells. 

IMG_1222-1
IonFlux Plate HT
(SINGLE CELLS)
PART# 910-0059

For lonFlux HT, lonFlux Mercury HT, IonFlux Mercury ULTRA.  Plates are standard SBS 384 wells. 

You can use your credit card to purchase IonFlux Plates directly.

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Contact us for more information about IonFlux systems. 

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