The IonFlux system's unique capabilities allow for the execution of otherwise very difficult assays. Its distinctive characteristics with fast in-plate solution exchange, continuous flow of external buffers, and best-in-class temperature control, permit not only fast screening of ion channel activity but also non-traditional assays such as analysis of cell membrane conductivity in the presence of external stressors. Below are some examples demonstrating the range of flexibility IonFlux systems provide:
Dr. Haiyang (David) Wei from Eurofins presented the abilities of data from IonFlux HT showing current stability, plate-to-plate reproducibility, agonists & antagonists effects, open channel block, PAM effects, binding kinetics, and recovery from desensitization. The presentation can be seen at the Ion Channel Retreat 2014 web site:
Presentation at the Ion Channel Retreat
Recently presented webinars focusing on assays ranging from screening of nicotinic compounds to toxin effects on potassium channels can be viewed at the Molecular Devices web site:
Webinars presented at Molecular Devices
Recent studies from University of Michigan Ann Arbor utilized an IonFlux system to monitor membrane permeability and disruption by analyzing overall conductance under whole-cell patch clamp:
- Detergent induction of HEK 293A cell membrane permeability measured under quiescent and superfusion conditions using whole cell patch clamp.
- Polyplex-induced cytosolic nuclease activation leads to differential transgene expression
Research at the University of Melbourne Australia focused on the N-terminus in assembly, trafficking and function of the GABA-C receptor:
Role of the ρ1 GABA-C Receptor N-Terminus in Assembly, Trafficking and Function.