Evaluating paralytic shellfish poisoning toxins'effects on seven human sodium channels
Paralytic shellfish poisoning (PSP) is a serious risk to human consumption of mollusks. Considering that the main target for these toxins is Nav channels, most studies screening for these compounds focused on binding assays instead of patch clamp. A study from Universidad de Santiago de Compostela in Spain focused on developing a screening assay for toxicity equivalence factors of PSP analogs using automated patch clamp (APC). Using an IonFlux system and established Nav cell lines, they showed the viability of using an APC in fulfilling deficiencies in other analytical methods and establishing a suitable setup for PSP screening. The study investigated Nav 1.1, 1.2, 1.3, 14, 1.5, 1.6 and 1.7 with nine PSP standards.
Evaluation of toxicity equivalent factors of paralytic shellfish poisoning toxins in seven human sodium channels types by an automated high throughput electrophysiology system.
Eva Alonso · Amparo Alfonso · Mercedes R. Vieytes · Luis M. Botana
Arch Toxicol. 2015 Jan 9.